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ProDetG TEV Protease

ProDetG TEV Protease

The ProDetG edition is tailored to uphold its high activity even in high concentrations of detergents (e.g. up to 2.5% DDM, 2.5% Triton X-100 or 6% CHAPS), setting it apart from the conventional NumacutTM variant. The N-terminal 6x His-Tag paves the way for easy and efficient removal of the Numacut from cleavage reactions by immobilized metal-ion affinity chromatography (IMAC). 

Regular price €239,00 EUR
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Product Specifications

Source: E. coli

Biological Activity: > 10,000 U/mL

Unit Definition: One Unit of Numacut TEV protease cleaves 80% of 3 µg control substrate containing a S amino acid residue at P1’ position in 1 h at 30°C and pH 7.5*. *Similar cleavage efficiencies observed for all amino acid residues at P1’ position except for proline.

Molecular Weight: 28.2 kDa

Formulation buffer: Lyophilized from buffer (20 mM Tris/HCl buffer, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, pH 7.5). No preservatives and carrier-free.

Purity: > 90% by SDS-PAGE analyses

Storage: Upon receipt, store the Numacut TEV protease at -20°C. The product is stable for at least six months. For application, reconstitute Numacut TEV protease by dissolving it with the provided reconstitution buffer. Reconstituted Numacut TEV protease should be stored at -20°C. Avoid thawing/freezing cycles.