TEV Protease wild type
TEV Protease wild type
Numaferm’s TEV Protease wild type is a highly specific enzyme derived from the Tobacco Etch Virus. It recognizes the sequence ENLYFQ↓S and cleaves most efficiently between the Q and S but can also accept small amino acids such as G, A, or H at the P1’ position. This enzyme is commonly used to remove affinity purification tags, such as GST- or His-tags, from fusion proteins.
Furthermore, Numaferm’s TEV Protease wild type includes beneficial mutations that enhance its specific activity, solubility, and stability. The N-terminal 6x His-Tag facilitates easy and efficient removal of the enzyme from cleavage reactions using immobilized metal-ion affinity chromatography (IMAC).
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Product Specifications
Source: E. coli
Biological Activity: > 10,000 U/mL
Unit Definition: One Unit of TEV protease cleaves 80% of 3 µg control substrate containing a S amino acid residue at P1’ position in 1 h at 30°C and pH 7.5
Molecular Weight: 28.2 kDa
Formulation buffer: Lyophilized from buffer (20 mM Tris/HCl buffer, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, pH 7.5). No preservatives and carrier-free.
Purity: > 90% by SDS-PAGE analyses
Storage: Upon receipt, store the TEV protease at -20°C. The product is stable for at least six months. For application, reconstitute the TEV protease by dissolving it with the provided reconstitution buffer. Reconstituted TEV protease should be stored at -20°C. Avoid thawing/freezing cycles.