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Numacut TEV Protease

Numacut TEV Protease

The Numacut™ TEV protease is the first platform capable of producing native proteins without leaving any additional residues. Evolved by artificial intelligence and directed evolution, its recognition site from the original TEV protease (ENLYFQ↓S) has been expanded to ENLYFQ↓X (where X can be any amino acid except proline). This new recognition site enables Numacut™ to cleave target peptides or proteins fused to a C-terminal ENLYFQ, independent of the N-terminal amino acid, without leaving additional residues.

Furthermore, Numacut™ features beneficial mutations that enhance specific activity, solubility, and stability, outperforming the wild-type enzyme. The N-terminal 6x His-Tag facilitates easy and efficient removal of Numacut™ from cleavage reactions via immobilized metal-ion affinity chromatography (IMAC).

Regular price €199,00 EUR
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Product Specifications

Source: E. coli

Biological Activity: > 10,000 U/mL

Unit Definition: One Unit of Numacut TEV protease cleaves 80% of 3 µg control substrate containing a S amino acid residue at P1’ position in 1 h at 30°C and pH 7.5*. *Similar cleavage efficiencies observed for all amino acid residues at P1’ position except for proline.

Molecular Weight: 28.2 kDa

Formulation buffer: Lyophilized from buffer (20 mM Tris/HCl buffer, 100 mM NaCl, 5 mM DTT, 1 mM EDTA, pH 7.5). No preservatives and carrier-free.

Purity: > 90% by SDS-PAGE analyses

Storage: Upon receipt, store the Numacut TEV protease at -20°C. The product is stable for at least six months. For application, reconstitute Numacut TEV protease by dissolving it with the provided reconstitution buffer. Reconstituted Numacut TEV protease should be stored at -20°C. Avoid thawing/freezing cycles.